Shanghai Yaxin Biotechnology Limited Company
We are professional supplier of Recombinant trypsin,Recombinant Carboxypeptidase B,Recombinant Enterokinase,Recombinant trypsin inhibitor,Sequencing Grade trypsin.
Business Type:Manufacturer
Country/Region:China
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Sequencing Grade Chymotrypsin
Catalog Number:SRCT10
CAS: 9004-07-3
EC: 3.4.21.1
Storage temperature: 2-8℃
Source: Sequencing grade chymotrypsin is a genetically engineered protein expressed in E.coli
Protein Sequence:The amino acid sequence of sequencing grade chymotrypsin is identical to human chymotrypsin
1. DESCRIPTION
Sequencing grade chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human chymotrypsin. Sequencing grade chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. The optimal pH is 7.0–9.0.
2. MAIN FEATURES
Source | Recombinant E. coli |
Appearance | White or off white powder |
Specific activity | ≥1500 USP units/mg pro. |
SDS-PAGE | Single major band |
SDS-PAGE (MW) | 24.0±2.4 kDa |
Purity(HPLC) | ≥ 95% |
3. RECOMMEND USAGE
Prepare 1-10mg/ml sequencing grade chymotrypsin with 50mM HAC.When digest the protein, the target protein was dissolved with 50 mM or 100 mM NH4HCO3, and then add a certain amount of enzyme.The ratio to aimed protein is 1:50 to 1:1000 (w/w) at 30℃.When the temperature is more than 30℃,enzyme will be self-cutting.
4. STABILITY OF STORAGE AND TRANSPORT
Stability of storage:Sequencing grade chymotrypsin lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolving with 50mM HAc, store at 2-8 ℃,or use within 2 days.If the solution is stored at -20 ℃ or below, it is stable within 24 months. The solution should avoid repeated freezing and thawing and placing at room temperature a long time.
Stability of transport: The product is stable by blue ice insulation transport.
5. PRODUCT ADVANTAGE
Sequence grade recombinant protein: high specificity,good stability.
Animal origin free: Sequencing grade chymotrypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory
requirements, and the production process is in full compliance with NSF ISO 9001: 2008 quality system and GMP guidelines.
Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
6. PRODUCT USAGE
Sequencing grade chymotrypsin can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.
7. RELATED PRODUCTS
Recombinant enterokinase
Sequencing grade trypsin
Sequencing grade V8 protease
Sequencing grade carboxypeptidase B
