Shanghai Yaxin Biotechnology Limited Company
We are professional supplier of Recombinant trypsin,Recombinant Carboxypeptidase B,Recombinant Enterokinase,Recombinant trypsin inhibitor,Sequencing Grade trypsin.
Business Type:Manufacturer
Country/Region:China
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Recombinant Human Trypsin
Catalog Number:RHT03
CAS: 9002-07-7
EC: 3.4.21.4
Storage temperature: 2-8℃
Source: Recombinant human trypsin is a genetically engineered protein expressed in E.coli
Protein Sequence:The amino acid sequence of recombinant human trypsin is identical to human pancreas-derived trypsin
1.DESCRIPTION
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. YaxinBio recombinant human trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence is identical to human pancreas-derived trypsin,with equivalent properties compared to native trypsin .The enzyme is inhibited by serine protease inhibitors, e.g. PMSF,soybean trypsin inhibitor and by metal chelating agents, e.g.EDTA.
2.MAIN FEATURES
Source | Recombinant E. coli |
Appearance | White or off white powder |
Specific activity | ≥3800 USP units/mg pro. |
Protein Content | ≥ 90% |
Purity(SDS-PAGE) | Single major band |
Molecular Weight(SDS-PAGE) | 24.0±2.4 kDa |
Purity(HPLC) | ≥ 95% |
UNIT DEFINITION：One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
3.RECOMMEND USAGE
Prepare 1-10mg/ml recombinant human trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.
4.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant human trypsin lyophilized should be stored under 2-8℃ in sealed container.
It is stable within 24 months.After dissolved with 1mM HCl , it should be stored under -20℃.
It is no activity loss after 10 times repeated freezing and thawing.
Stability of transport: The product is stable by blue ice insulation transport.
5.PRODUCT ADVANTAGE
l Animal origin free: Recombinant human trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
l Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
l High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
l Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
l Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
l Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
6.PRODUCT USAGE
Recombinant human trypsin is an endopeptidase that can be used for lysine and arginine C-terminal cleavage of peptide bonds to cleave macromolecules into small peptides. Recombinant human trypsin is widely used in a variety of biotechnological processes, such as: cell culture cell separation of various tissues; degradation of denatured proteins; enzymatic hydrolysis, sequencing; stem cell therapy; tumor cell therapy.
7.RELATED PRODUCTS
Recombinant carboxypeptidase B
Sequencing grade trypsin
Recombinant trypsin
Recombinant enterokinase
